Immunocytolocalization of CDSP 32 and CDSP 34, two chloroplastic drought-induced stress proteins in Solanum tuberosum plants |
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| Affiliation: | 1. CAS Key Laboratory of Computational Biology, Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;3. Department of Biochemistry, Department of Plant Biology, and Center of Biophysics and Quantitative Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA;1. Department of Plant & Microbial Biology, North Carolina State University, Raleigh, NC, 27695, United States;2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Beijing, 100091, China;3. Department of Plant Sciences, University of California, Davis, California 95616;4. Department of Botany, University of Wisconsin, Madison, Wisconsin 53706;1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Shanda South Road 27, Jinan, Shandong 250100, China;2. RCBB, College of Resources and Environment, Southwest University, Tiansheng Road 2, Beibei Dist., 400716 Chongqing, China |
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| Abstract: | Two chloroplastic proteins, named CDSP 32 and CDSP 34 for chloroplastic drought-induced stress protein of 32 and 34 kDa, were previously shown to be substantially synthesized in Solanum tuberosum plants subjected to water deficit. We investigated the localization of CDSPs in leaf chloroplasts from control and wilted potato plants using immunocytochemistry. Observation of electron micrographs did not reveal any important change in plastid structures of drought-stressed plants, except an increased number and a larger size of plastoglobuli. In well-watered plants, very little labeling corresponding to CDSP 32 was detected. Consecutively to water stress, a higher abundance of CDSP 32 was revealed, the protein being exclusively localized in the stroma. Immunocytochemical data indicated the presence of some CDSP 34 protein in well-watered plants and confirmed its accumulation upon water deficit. CDSP 34 was found to be preferentially associated with stromal lamellae thylakoids, but some protein was revealed in the stroma. No association of CDSP 34 with grana and plastoglobuli was noticed in chloroplasts from control and stressed plants. |
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