Stimulation of cell wall biosynthesis and structural changes in response to cytokinin- and elicitor-treatments of suspension-cultured Phaseolus vulgaris cells |
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| Institution: | 1. Division of Biochemistry, School of Biological Sciences, Royal Holloway and Bedford New College, University of London, Egham Surrey, TW20 0EX, UK;2. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland;1. Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;2. Interfaculty Institute of Biochemistry (IFIB), Eberhard Karls University, Tübingen, Germany;3. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;4. Center for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen, Germany;5. Center of Excellence in Medicinal Plant Metabolites, Tarbiat Modares University, Tehran, Iran;1. Water Pollution Research Department, National Research Centre, 33 El Bohouth st. (El Tahrir st. former)-Dokki-Giza-Egypt, P.O. 12622, Egypt;2. Faculty of Science, Fayoum University, Fayoum, Egypt;3. Egypt Nanotechnology Research Center, Cairo University, Elsheikh Zayed Campus, Giza, Egypt;4. Arab Academy for Science, Technology, and Maritime Transport, Smart Village Campus, Giza, Egypt;5. Lehrstuhlfür Technische Chemie II and Centre for Water and Environmental Research (ZWU), Universität Duisburg-Essen, 45117 Essen, Germany;6. Faculty of Science, Chemistry Department, Ain Shams University, Cairo, Egypt;1. School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland;2. UCD Centre for Plant Science, Ireland;1. DokaGene Ltd., Rogachevo, Moscow Region, Russia;2. Lomonosov Moscow State University, Leninsky Gory, Moscow 119992, Russia;3. James Hutton Institute, Invergowrie, DD2 5DA Dundee, UK |
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| Abstract: | Qualitative sugar flux into cell wall polysaccharides has been determined for two model systems. The first, treatment of suspension-cultured French bean (Phaseolus vulgaris L.) cells with an increase in the cytokinin/auxin ratio and in the concentration of sucrose, models some aspects of differentiation. Wall changes are characterised by up to a five-fold increase in thickness due to the laying down of extra wall material. Sugar flux following labelling of cells with 14C]-sucrose was examined during the period of maximum extractable catalytic activities of the enzymes of sugar nucleotide conversion determined previously. Increased secretion was observed in all major groups of polysaccharides, particularly the cellulosic fraction. Analysis of the sugars in the hemicellulosic fraction indicated that the newly synthesised polysaccharide was most probably xylan. It was confirmed by immunolocalisation of xylan in these walls. This treatment thus increases incorporation into the wall of components characteristic of secondary wall. In the second system, which models the defence response, suspension cultures were treated with an elicitor from the walls of Colletotrichum lindemuthianum. Again, sugar flux was determined by labelling cells with 14C]-sucrose and examined during the period determined previously of maximum extractable catalytic activities of the enzymes of sugar nucleotide conversion. Increased secretion into unextractable polymers was the major change and was consistent with the occurrence of oxidative processes leading to immobilisation of some wall components. Callose, a polysaccharide characteristic of the defence response was immunolocalised in these walls but not in those of control cells. |
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