Identification and endothelin-induced activation of multiple extracellular signal-regulated kinases in aortic smooth muscle cells. |
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Authors: | T Yoshimasa K Nakao S Suga I Kishimoto Y Kiso H Imura |
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Affiliation: | Department of Medicine, Kyoto University School of Medicine, Japan. |
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Abstract: | In order to explore intracellular signaling pathways of the mitogenic action of endothelin (ET), we examined the effect of ET on activities of extracellular signal-regulated kinases (ERKs) in rat aortic smooth muscle cells (SMCs). Treatment of rat aortic SMCs with ET-1 increased kinase activities toward myelin basic protein (MBP). Both 43- and 41-kDa proteins were activated when kinase assays were done in MBP-containing polyacrylamide gels after SDS-PAGE. These proteins were identified as ERK1 and ERK2 with immunoprecipitation and immunoblotting using antipeptide antibodies, respectively. These results indicate that ERKs mediate signal transduction by ET. |
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