内皮素-2促进体外培养的绵羊皮肤黑色素细胞增殖及黑色素生成

内皮素（endothelin,ET）及其受体在黑色素细胞成熟分化时起着有效的促进作用.然而，内皮素-2（ET-2）在黑色素生成的作用方面，还处于争论中或报道不一致.在此,我们研究ET-2对体外培养的绵羊皮肤黑色素细胞增殖和黑色素生成的影响.比较实验组ET-2（1,10,100 nmol/L）与空白对照组，通过MTT法和Ando等的方法检测出黑色素细胞的增殖率和黑色素含量显著增加.荧光定量PCR和Western 印迹分别检测出内皮素受体B(Bdnrb)；酪氨酸激酶受体(Kit)；酪氨酸酶(Tyr)；酪氨酸相关蛋白-1(Tyrp-1)基因的mRNA水平及蛋白水平的相对表达量显著增加.这些数据表明,ET-2可能促进绵羊的皮肤黑色素细胞增殖和黑色素生成.

Endothelin-2 Promotes Ovine Skin Melanocytes Proliferation and Melanin Melanogenesis in Vitro

Endothelin (ET) and its receptor have been shown to play roles in the melanocyte maturity. However, the reports on the function of ET-2 in melanogenesis are debated. Herein, we investigated the effect of ET-2 on ovine skin melanocyte proliferation and melanogenesis. MTT method and Ando methods showed that the cell proliferation rates and melanin levels were significantly increased in melanocytes treated by ET-2 (1 to 100 nmol/L) compared with the control (untreated) cells. Furthermore, real-time quantitative PCR and Western blotting revealed that the levels of both mRNA and protein of the Bdnrb, Tyr, Kit and Tyrp-1 genes were significantly increased. These data suggest that ET-2 might promote proliferation of ovine skin melanocytes and melanogenesis.