Adherence ofStaphylococcus epidermidis to pharyngeal epithelial cells mediated by lipoteichoic acid |
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Authors: | Prof Tulsi D Chugh George M Bahr Sahar A Essa Gary J Burns |
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Institution: | (1) Department of Biochemistry, Faculty of Medicine, Kuwait University, Kuwait;(2) Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait |
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Abstract: | Prior treatment of pharyngeal epithelial cells (PEC) with lipoteichoic acid (LTA) derived fromStaphylococcus epidermidis produced a marked inhibition of adherence of the homologous strain and two heterologous strains. The inhibition was dose dependent and saturable with 100 µg/ml of LTA. However, pretreatment of PEC with deacylated LTA did not block the adherence of the three strains tested. A similar but less marked blocking effect on the adherence ofS. epidermidis to PEC was also observed with LTAs derived fromS. aureus andStreptococcus pyogenes. On treatment of bacteria with substances capable of binding to LTA, such as polyclonal mouse anti-LTA antibodies or with human albumin, a marked inhibition of bacterial adherence was observed. Immunofluorescence studies showed that anti-LTA antiserum bound readily to the surface of bacterial cells. These findings provide clear evidence that the lipid component of LTA located on the bacterial surface is centrally involved in the adherence ofS. epidermidis to human mucosal cells. |
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