Development of complex enzymatic preparations of pactinases and celulases for sugar beet marc digestion |
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Authors: | E V Bushina A M Rozhkova I N Zorov A D Satrutdinov A O Bekkarevich A V Koshelev O N Okunev A P Sinitsyn |
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Institution: | (1) Department of Spirit and Yeast Technology, Institute of Fermentation Technology and Microbiology, Technical University of Lodz, Wolczanska 171/173, 90-924 Lodz, Poland |
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Abstract: | Complex enzymatic preparations demonstrating activities homologous to pectinlyase A and heterologous to endo-1,4-β-glucanase from Penicilliumverruculosum and β-glycosidase from Aspergillusniger have been obtained on the basis of recombinant strains of the fungus Penicilliumcanescens. Two approaches were utilized: development of an enzymatic preparation on the basis of a new strain, which produced all three enzymes, and development of an enzymatic preparation via combined cultivation of three strains, each of which produced one of the enzymes. |
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