Distribution of S-haplotypes and relationship with self-incompatibility in Brassica oleracea. 2. In varieties of broccoli and romanesco |
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Authors: | V Ruffio-Châble J P Le Saint T Gaude |
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Institution: | (1) INRA, Station d’Amélioration des Plantes, BP 29, 35653 Le Rheu Cedex, France Fax: +33 299 28 5120 E-mail: vruffio@rennes.inra.fr, FR;(2) INRA, Laboratoire d’Amélioration des Plantes Maraichères, Kerdévez, 29250 Plougoulm, France, FR;(3) Reproduction et Développement des Plantes, UMR 9938 CNRS-INRA-ENSL, Ecole Normale Supérieure de Lyon, 46 Allée d’Italie, 69364 Lyon Cedex 07, France, FR |
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Abstract: | The self-incompatibility (SI) character in Brassica is controlled by the S locus which contains several genes. One of them, the SLG (S Locus Glycoprotein) gene encodes a soluble glycoprotein expressed in the stigma. We used antibodies directed against SLGs
and a combination of isoelectric focusing (IEF) and immunoblotting methods to identify S haplotypes, the allelic forms of the S locus, in commercial and open-pollinated varieties of broccoli and romanesco. We found 23 class-I and three class-II S haplotypes among the 199 plants analysed. Nevertheless, for a few plants, SLGs were not detected by the antibodies and these
plants, designated Hw for “white pattern” haplotypes, were apparently homozygous at the S locus. Diallel crosses between Hw plants revealed the existence of four different Hw haplotypes. Several hypotheses are discussed
to explain the non-recognition of the SLG products in these Hw haplotypes. The data of the present study were compared with
those obtained in a previous investigation carried out on cauliflower. As in cauliflower, we observed a high frequency of
the sx haplotype and a great variability in the strength of the SI phenotype for sx plants (in the homozygous or heterozygous
state). For both broccoli and romanesco, about 50% of the plants presented a SI phenotype strong enough to be exploited for
hybrid production.
Received: 27 July 1998 / Accepted: 5 August 1998 |
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Keywords: | Self-incompatibility S-haplotype S-Locus glycoprotein Immunochemical analysis Brassica oleracea |
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