Determinants for Membrane Association of the Hepatitis C Virus NS2 Protease Domain |
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| Authors: | Christian M. Lange Pantxika Bellecave Viet Loan Dao Thi Huong T. L. Tran Fran?ois Penin Darius Moradpour Jér?me Gouttenoire |
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| Affiliation: | aDivision of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland;bInstitut de Biologie et Chimie des Protéines, Bases Moléculaires et Structurales des Systèmes Infectieux, UMR 5086, CNRS, Labex ECOFECT, University of Lyon, France |
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| Abstract: | Hepatitis C virus (HCV) nonstructural protein 2 (NS2) is required for HCV polyprotein processing and particle assembly. It comprises an N-terminal membrane domain and a C-terminal, cytosolically oriented protease domain. Here, we demonstrate that the NS2 protease domain itself associates with cellular membranes. A single charged residue in the second α-helix of the NS2 protease domain is required for proper membrane association, NS2 protein stability, and efficient HCV polyprotein processing. |
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