In Vitro Bactericidal and Bacteriolytic Activity of Ceragenin CSA-13 against Planktonic Cultures and Biofilms of Streptococcus pneumoniae and Other Pathogenic Streptococci |
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| Authors: | Miriam Moscoso María Esteban-Torres Margarita Menéndez Ernesto García |
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| Affiliation: | 1. Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.; 2. Departamento de Química-Física Biológica, Instituto Química-Física Rocasolano, CSIC, Madrid, Spain.; 3. CIBER de Enfermedades Respiratorias (CIBERES), Mallorca, Illes Balears, Spain.; Rockefeller University, United States of America, |
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| Abstract: | Ceragenin CSA-13, a cationic steroid, is here reported to show a concentration-dependent bactericidal/bacteriolytic activity against pathogenic streptococci, including multidrug-resistant Streptococcus pneumoniae. The autolysis promoted by CSA-13 in pneumococcal cultures appears to be due to the triggering of the major S. pneumoniae autolysin LytA, an N-acetylmuramoyl-L-alanine amidase. CSA-13 also disintegrated pneumococcal biofilms in a very efficient manner, although at concentrations slightly higher than those required for bactericidal activity on planktonic bacteria. CSA-13 has little hemolytic activity which should allow testing its antibacterial efficacy in animal models. |
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