Genetic Mutation Analysis of Human Gastric Adenocarcinomas Using Ion Torrent Sequencing Platform |
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| Authors: | Zhi Xu Xinying Huo Hua Ye Chuanning Tang Vijayalakshmi Nandakumar Feng Lou Dandan Zhang Haichao Dong Hong Sun Shouwen Jiang Guangchun Zhang Zhiyuan Liu Zhishou Dong Baishuai Guo Yan He Chaowei Yan Lu Wang Ziyi Su Yangyang Li Dongying Gu Xiaojing Zhang Xiaomin Wu Xiaowei Wei Lingzhi Hong Yangmei Zhang Jinsong Yang Yonglin Gong Cuiju Tang Lindsey Jones Xue F. Huang Si-Yi Chen Jinfei Chen |
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| Affiliation: | 1. Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.; 2. San Valley Biotechnology Incorporated, Beijing, China.; 3. Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.; National Cancer Center, Japan, |
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| Abstract: | Gastric cancer is the one of the major causes of cancer-related death, especially in Asia. Gastric adenocarcinoma, the most common type of gastric cancer, is heterogeneous and its incidence and cause varies widely with geographical regions, gender, ethnicity, and diet. Since unique mutations have been observed in individual human cancer samples, identification and characterization of the molecular alterations underlying individual gastric adenocarcinomas is a critical step for developing more effective, personalized therapies. Until recently, identifying genetic mutations on an individual basis by DNA sequencing remained a daunting task. Recent advances in new next-generation DNA sequencing technologies, such as the semiconductor-based Ion Torrent sequencing platform, makes DNA sequencing cheaper, faster, and more reliable. In this study, we aim to identify genetic mutations in the genes which are targeted by drugs in clinical use or are under development in individual human gastric adenocarcinoma samples using Ion Torrent sequencing. We sequenced 737 loci from 45 cancer-related genes in 238 human gastric adenocarcinoma samples using the Ion Torrent Ampliseq Cancer Panel. The sequencing analysis revealed a high occurrence of mutations along the TP53 locus (9.7%) in our sample set. Thus, this study indicates the utility of a cost and time efficient tool such as Ion Torrent sequencing to screen cancer mutations for the development of personalized cancer therapy. |
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