Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals |
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| Authors: | Satyajit D. Sarker Lutfun Nahar Yashodharan Kumarasamy |
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| Affiliation: | aSchool of Biomedical Sciences, University of Ulster at Coleraine, Cromore Road, Coleraine BT52 1SA, Co. Londonderry, Northern Ireland, UK;bDepartment of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK;cSchool of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen AB10 1FR, Scotland, UK |
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| Abstract: | The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products. |
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| Keywords: | Antibacterial assay Resazurin Plant extract Serial dilution |
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