| 离子交换法分离发酵液中鸟苷和肌苷 |
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| 引用本文： | 刘辉,武改红,王颖,周昌平,路颖,陈宁. 离子交换法分离发酵液中鸟苷和肌苷[J]. 生物学杂志, 2005, 22(3): 41-43 |
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| 作者姓名： | 刘辉 武改红 王颖 周昌平 路颖 陈宁 |
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| 作者单位： | 天津科技大学食品科学与生物工程学院,天津,300222 |
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| 基金项目： | 天津高等学校科技发展基金项目(20031205) |
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| 摘 要： | 乌苷发酵液中乌苷与副产物肌苷的分离对乌苷工业有重要的意义。对乌苷和肌苷的分离条件进行研究，得到了最佳分离条件：采用Hd-8树脂，树脂床高度为4cm，前120mL采用0．05mol／L盐酸洗脱收集肌苷，120mL至240mL采用0．74mol／L pH5．0醋酸钠缓冲液洗脱收集乌苷，可以获得很好的效果。
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| 关 键 词： | 离子交换法 分离 发酵液 鸟苷 肌苷 |
| 文章编号： | 1008-9632(2005)03-0041-03 |
| 修稿时间： | 2004-03-28 |
Studies on the separation of guanosine and inosine in fermentation broth by ion exchange |
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| LIU Hui,WU Gai-hong,WANG Ying,ZHOU Chang-ping,LU Ying,CHEN Ning. Studies on the separation of guanosine and inosine in fermentation broth by ion exchange[J]. Journal of Biology, 2005, 22(3): 41-43 |
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| Authors: | LIU Hui WU Gai-hong WANG Ying ZHOU Chang-ping LU Ying CHEN Ning |
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| Abstract: | It is very important in the guanosine industry that guanosine must be completely separated from byproduct-inosine.The condition of analysis and separation of guanosine and inosine was studied.The optimal condition was finally obtained.The resin was HD-9=8.Its bed height was 4 centimeter.The gradient eluate solution was 0.05mol/L HCL for the first 120mL eluant of insoine and 0.74mol/L NaAc buffer(pH5.0)for the eluant from 120mL to 400mL of guanosine.The method was useful and repeated well. |
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| Keywords: | inosine guanosine ion exchange separation |
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