The high-energy diet affecting development of diabetes symptoms in Zucker diabetic fatty rats |
| |
Authors: | Marcela Capcarova Anna Kalafova Marianna Schwarzova Marta Soltesova Prnova Karol Svik Monika Schneidgenova Lukas Slovak Ivana Bovdisova Robert Toman Viktoria Lory Stefan Zorad |
| |
Affiliation: | 1.Department of Animal Physiology, Faculty of Biotechnology and Food Sciences,Slovak University of Agriculture in Nitra,Nitra,Slovak Republic;2.Department of Human Nutrition, Faculty of Agrobiology and Food Resources,Slovak University of Agriculture in Nitra,Nitra,Slovak Republic;3.Institute of Experimental Pharmacology and Toxicology,Slovak Academy of Science,Bratislava,Slovak Republic;4.Department of Veterinary Disciplines, Faculty of Agrobiology and Food Resources,Slovak University of Agriculture in Nitra,Nitra,Slovak Republic;5.Institute of Experimental Endocrinology,Slovak Academy of Science,Bratislava,Slovak Republic |
| |
Abstract: | The appropriate animal model of diabetes mellitus type 2 is Zucker diabetic fatty (ZDF) rats. The goal of this study was to analyse the effect of chronic high-energy diet on diabetes mellitus (DM) complications in ZDF rats. Male ZDF rats (n?=?20) and their lean controls (non-diabetic, n?=?10) in the age of 3 months were involved in the experiment. Rats were provided with water and diet on ad libitum base. Animals were divided into three groups as follows: lean untreated rats (C) fed by KKZ-P/M (10 MJ/kg), obese rats fed by KKZ-P/M (10 MJ/kg, E1) and obese rats fed by enriched high energy diet (E2, enriched KKZ-P/M, 20 MJ/kg). Glucose, ketones levels, the consumption of feed, water and the live weight was measured weekly during the whole experiment. At the end of the experiment rats were anesthetized and selected haematological parameters were measured. ZDF rats in E1 and E2 group developed obesity, hyperglycaemia, non-insulin dependent diabetes, aggravations in haematological parameters and accumulation of sorbitol in sciatic nerve and lens of rats. High-energy diet immediately induced hyperglycaemia followed by accelerating the secondary symptoms of diabetes complications expressed by disturbed haematology parameters. High-energy diet caused ketoacidosis what meant two cases of death. Extended research on diabetes is needed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|