碱性成纤维细胞生长因子对生长板软骨细胞增殖与分化的影响

目的:探讨碱性成纤维细胞生长因子(bFGF)对生长板软骨细胞增殖和分化的作用。方法:分离并在低血清条件下培养兔生长板软骨细胞。采用改良MTT法检测细胞增殖倍数;羟脯氨酸法测定软骨细胞胶原产量;酶动力学方法测定碱性磷酸酶(ALP)活性。结果:bFGF浓度在5-100ng／ml范围内可以促进软骨细胞增殖,并以25ng／ml刺激时效果最为显著。当bFGF浓度高于25ng／ml时,抑制软骨细胞的胶原合成;当高于1ng／ml时,抑制碱性磷酸酶活性。结论:bFGF刺激生长板软骨细胞增殖,并在较高浓度时抑制生长板软骨细胞的分化。

The Effect of bFGF on the Proliferation and Diffrentiation of Growth-plate Chondrocytes

Objectives: To study the effect of basic fibroblast growth factor (bFGF) on the proliferation and differentiation in cultured growth-plate chondrocytes. Methods: Growth-plate chondrocytes were isolated from rabbit costal cartilage and cultured under low-serum condition in monolayers. Cell growth was detected by the improved MTT; the production of collagen was estimated according to the hydroxyproline content; ALP activity was measured by modified enzyme dynamical method. Result: The cultured chondrocytes in medium with bFGF at concentrations of 5 -100ng/ml were significantly more than those in control group, which reached the peak at the concentration of 25ng/ml. The synthesis of collagen was inhibited by bFGF at the concentration of above 25ng/ml. The activity of ALP was suppresseded by bFGF at the concentration of above 1ng/ml. Conclusion: bFGF stimulated the proliferation of growth-plate chondrocytes obviously, but inhibited the differentiation of growth-plate chondrocytes at high concentrations.