Formation of Extracellular Enzyme Systems during the Growth of Geotrichum candidum3C on Cell Walls Isolated from Cereal Grain Coats |
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Authors: | Rodionova N A Dubovaya N V Martinovich L I Bezborodov A M |
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Institution: | (1) Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia;(2) Moscow State University of Food Technologies, Moscow, 125080, Russia |
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Abstract: | The activities of extracellular systems of hemicellulases, pectinases, and cellulases was studied during a 72-h cultivation of Geotrichum candidum3C. The culture was grown on a medium containing 3% cell walls isolated from wheat grain coats, which served as the sole carbon source. Enzymes catalyzing the degradation of pectin substances (beet pectin, -L-arabinan, and 1,4- -D-galactan), as well as -D-galactosidase and -L-arabinofuranosidase involved in their hydrolysis, were formed first (4 h after the beginning of cultivation). Enzymes hydrolyzing 4-O-methyl- -D-glucurono- -D-xylan and sodium carboxymethyl xylan were also found in the culture liquid after 4 h of fungal growth. The contents of pectin-degrading and xylanolytic enzymes reached their maximum levels after 52–56 and 72 h of growth, respectively. Cellulolytic enzymes were detected after 8–28 h of cultivation. Enzymes degrading -D-galacto- -D-mannan were found 24 h after the beginning of growth; their content was maximum after 72 h of cultivation. |
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