Placental Transfer of Lactate,Glucose and 2-deoxyglucose in Control and Diabetic Wistar Rats |
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Authors: | Chris R Thomas Beryl B Oon Clara Lowy |
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Institution: | 1. Department of Medicine, Guys Kings and St. Thomas School of Medicine, St. Thomas'' Hospital, Lambeth Palace Road, London, SE1 7EH, UK, |
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Abstract: | Placental transfer of lactate, glucose and 2-deoxyglucose
was examined employing the in situ perfused
placenta. Control and streptozotocin induced diabetic
Wistar rats were infused with U14C]-glucose and
3H]-2-deoxyglucose (2DG). The fetal side of the placenta
was perfuseci with a cell free medium and glucose
uptake was calculated in the adjacent fetuses.
Despite the 5-fold higher maternal plasma glucose
concentration in the diabetic dams the calculated
fetal glucose metabolic index was not significantly
different between the 2 groups. Placental blood flow
was reduced in the diabetic animals compared with
controls but reduction of transfer of U14C]-glucose and 3H]-2-deoxyglucose and endogenously derived
14C]-Lactate to the fetal compartment, could not be
accounted for by reduced placental blood flow
alone. There was no significant net production or
uptake of lactate into the perfusion medium that
had perfused the fetal side of the placenta in either
group. The plasma lactate levels in the fetuses adjacent
to the perfused placenta were found to be higher
than in the maternal plasma and significantly
higher in the fetuses of the diabetic group compared
with control group. In this model the in situ perfused
placenta does not secrete significant quantities
of lactate into the fetal compartment in either
the control or diabetic group. |
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