Nickel,a component of factor F 430 from Methanobacterium thermoautotrophicum |
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Authors: | Diekert Gabriele Klee Birte Thauer Rudolf K |
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Institution: | (1) Fachbereich Biologie/Mikrobiologie, Philipps-Universität Marburg, D-3550 Marburg, Germany |
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Abstract: | Methanobacterium thermoautotrophicum, growing on medium supplemented with 2 mol 63NiCl2/l, was found to take up 1.2 mol 63Ni per g cells (dry weight). More than 70% of the radioisotope was incorporated into a compound, which dissociated from the protein fraction after heat treatment, was soluble in 70% acetone, and could be purified by chromatography on QAE-Sephadex A-25, Sephadex G-25, and DEAE cellulose. The purified 63Ni labelled compound had an absorption spectrum and properties identical to those of factor F
430 and is therefore considered to be identical with factor F
430.Factor F
430, a compound of molecular weight higher than 1000 with an absorbance maximum at 430 nm, has recently been purified from Methanobacterium thermoautotrophicum (Gunsalus and Wolfe, 1978). The structure and function of this compound are not yet known. |
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Keywords: | Methanobacterium thermoautotrophicum Nickel Factor F
430 |
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