| 小溪自然保护区嗜盐及耐盐菌抗菌活性筛选及生物学特征 |
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| 引用本文： | 陈奇辉,张丽,刘祝祥,黄苛,熊利芝,贺建武,陈义光.小溪自然保护区嗜盐及耐盐菌抗菌活性筛选及生物学特征[J].微生物学杂志,2010,30(4):7-12. |
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| 作者姓名： | 陈奇辉 张丽 刘祝祥 黄苛 熊利芝 贺建武 陈义光 |
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| 作者单位： | 吉首大学生物资源与环境科学学院,植物资源保护与利用湖南省高校重点实验室,湖南,吉首,416000 |
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| 基金项目： | 国家自然科学基金，吉首大学项目 |
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| 摘 要： | 以8个敏感菌株(3株革兰阳性菌、3株革兰阴性菌和2株真菌)作为指示菌,采用管碟法对分离自湖南小溪自然保护区普通非盐环境(ordinary non-saline environment)土样中的114株嗜盐及耐盐细菌(含放线菌)进行抗菌活性筛选,并对抗菌活性较强的菌株进行了基于16S rRNA基因序列的系统发育分析和生物学特性研究。结果显示,74株受试菌株的发酵产物具有抗菌活性,阳性率为64.9%。综合分析形态特征、生理生化特征和基于16S rRNA基因序列的系统发育分析数据,表明9株具有较强抗菌活性的菌株分别属于Bacillus属(JSM 081049、JSM 082021-1、JSM 082056、JSM 082080、JSM 082081-1、JSM 082097)、Arthrobacter属(JSM082018)、Brachybacterium属(JSM082044)和Streptomyces属(JSM082030)。
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| 关 键 词： | 普通非盐环境 嗜盐及耐盐菌 抗菌活性 生物学特性 系统发育分析 |
Screening and Biological Characteristics of Antibiotic Active Halophilic and Halo-tolerant Bacteria from Xiaoxi National Natural Reserve |
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| CHEN Qi-hui,ZHANG Li,LIU Zhu-xiang,HUANG Ke,XIONG Li-zhi,HE Jian-wu,CHEN Yi-guang.Screening and Biological Characteristics of Antibiotic Active Halophilic and Halo-tolerant Bacteria from Xiaoxi National Natural Reserve[J].Journal of Microbiology,2010,30(4):7-12. |
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| Authors: | CHEN Qi-hui ZHANG Li LIU Zhu-xiang HUANG Ke XIONG Li-zhi HE Jian-wu CHEN Yi-guang |
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| Institution: | CHEN Qi-hui,ZHANG Li,LIU Zhu-xiang,HUANG Ke,XIONG Li-zhi,HE Jian-wu,CHEN Yi-guang(Key Lab.for Cons.& Util.of Plant Res.,Coll.of Biol.and Envir.Sci.,Jishou Uni.,Jishou,Hunan 416000) |
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| Abstract: | Antimicrobial activities of fermentation broth of 114 halophilic and halo-tolerant bacteria(including Actinomyces) isolated from ordinary non-saline soil collected from Xiaoxi National Natural Reserve,Hunan Province,was screened using the cylinder-plate method with 8 sensitive strains as indicator microbes(three Gram-positive bacteria,three Gram-negative bacteria and two fungi).The results showed that,among 114 strains tested,74 were positive in antimicrobial activity(positive rate at 64.9%),among them 9 st... |
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| Keywords: | ordinary non-saline environment halophilic and halo-tolerant bacteria antimicrobial activity biological characteristics phylogenetic analysis |
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