Determination of deoxycorticosterone and aldosterone in biological samples by gas chromatography with electron capture detection |
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| Authors: | J P Rapp K B Eik-Nes |
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| Affiliation: | 1. Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China;2. Department of Community Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada;3. Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada;1. Dallas Diabetes Research Center at Medical City, Dallas, TX, USA;2. Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy |
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| Abstract: | Gas chromatography with electron capture detection was used for quantitation of deoxycorticosterone and aldosterone from biological samples. Purification of samples prior to final quantitation on the gas chromatograph was done by paper chromatography, derivative formation, and then thin-layer chromatography. The derivatives used which were sensitive to electron capture detection were deoxycorticosterone acetae and aldosterone γ-lactone. As little as 0.02 μg of either deoxycorticosterone or aldosterone in the original sample could be quantitated. The methods were applied to simulated samples, rabbit adrenal venous blood. and adrenal incubates. Specific activities of deoxycorticosterone and aldosterone from adrenals incubated with progesterone-4-C11 were obtainable with the methods developed. |
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