植物细胞凋亡的ELISA检测(英文)

本文利用ＴＵＮＥＬ方法在单个细胞水平上对苯胺灵诱导的玉米根尖细胞死亡进行了检测。结果表明，一定浓度的苯胺灵能诱导玉米根尖细胞发生主动性的细胞凋亡，具有细胞凋亡典型的形态和生化特征即细胞核浓缩并形成核碎片、染色质边缘化及ＤＮＡ特异片段化等（Ｆｉｇ．１）。首次利用ＥＬＩＳＡ方法在群体水平上对这种细胞凋亡进行了进一步检测。结果表明：动物细胞研究常用的ＥＬＩＳＡ方法同样也适合用于植物细胞凋亡的检测。在凋亡前期，随着凋亡过程的进行，细胞质中的ＯＤ值逐渐上井（Ｆｉｇ．２）。剂量实验表明，０．２ｍｇ／ｍＬ苯胺灵最适合于诱导细胞凋亡（Ｆｉｇ．３）。

ELISA Assays for Detection of Apoptosis in Plant Cells

This paper presents the results of the ELISA and TUNEL assays for the detection of cell death in maize root tips. The results indicates that the active apoptosis process can be induced in the root-tip cells of maize by isopropyl carbanilate (IPC) at moderate concentrations, and characterized by morphological and biochemical characteristics, including DNA fragmentation, nuclear condensation and chromatin marginalization (Fig.1). The ELISA technique was used for the first time for detection of apoptosis in plant system, and the results indicate it is available in plant cells. OD values of cytoplasm obtained from ELISA rose gradually during early apoptotic period and then declined (Fig.2). The results of dosage-dependent tests indicate that 0.2 mg/mL IPC is proper for inducing apoptosis in maize root cells.