Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions |
| |
Authors: | Zhang Kexiong Wang Qiaoqiao Xie Yanhua Mor Gil Sega Emanuela Low Philip S Huang Yingqun |
| |
Institution: | Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06511, USA. |
| |
Abstract: | We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells. |
| |
Keywords: | siRNA delivery gene therapy folate receptor |
本文献已被 PubMed 等数据库收录! |
|