ERIC-PCR技术在李斯特氏菌种、菌株鉴定中的应用

应用肠杆菌基因间重复一致序列聚合酶链反应技术（ERIC-PCR）对李斯特氏菌基因组DNA进行分析，结果显示，李斯特氏菌种间DNA指纹图谱带型差异较大；单核细胞增生性李斯特氏菌株间及相同血清型不同来源的菌株，其DNA指纹图谱带型也有明显差异。在单核细胞增生性李斯特氏菌各株的DNA指纹图谱中发现1600bp的种专一性扩增带。结果表明，ERIC-PCR技术可用于李斯特氏菌种、菌株的鉴定及进一步分型研究。 Abstract:Enterobacteia repetitive intergenic consensus sequences-based PCR(ERIC-PCR) was used to generate DNA fingerprints for Listeria spp.We got the specific profiles with ERIC-PCR technique that enables to identify Listeria species and the L.monocytogenes strains of different sterotype,and the same sterotype of L.monocytogenes from different sources also could be identified.Moreover,the species-spcific 1600bp DNA fragment was obtained from the fingerprint of L.monocytogenes.The study indicates that ERIC-PCR technique can be used in the identification of Listeria species and strains and its further typing,which is simple and quickly.

ERIC-PCR on Identification of Listeria Species and Strain

Enterobacteria repetitive intergenic consensus sequences-based PCR(ERIC-PCR) was used to generate DNA fingerprints for Listeria spp. We got the specific profiles with ERIC-PCR technique that enables to identify Listeria species and the L.monocytogenes strains of different stereotype, and the same stereotype of L. monocytogenes from different sources also could be identified. Moreover,the species-specific 1600 bp DNA fragment was obtained from the fingerprint of L. monocytogenes. The study indicates that ERIC-PCR technique can be used in the identification of Listeria species and strains and its further typing, which is simple and quickly.