Apoptosis of murine BW 5147 thymoma cells induced by dexamethasone and gamma-irradiation |
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| Authors: | I I Kruman N P Matylevich I P Beletsky V N Afanasyev S R Umansky |
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| Institution: | Institute of Biological Physics, USSR Academy of Sciences, Moscow Region. |
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| Abstract: | The mode and the kinetics of the death of T-thymoma cells upon dexamethasone treatment and gamma-irradiation (10Gy) have been studied using flow cytometry and biochemical analysis. It has been shown that the hormone and gamma-irradiation induce cell death by apoptosis. In both cases the cells are initially blocked in G2/M and die only after overcoming the blockage and cytokinesis. A short exposure to dexamethasone results in a cytostatic effect, whereas a cytotoxic effect is absent. Reducing serum concentration to 2% causes more rapid death both following gamma-irradiation and dexamethasone. These results are discussed in relation to cell death and proliferation. |
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