Phenotypic expression of epigenetic determinant [ISP
+] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations |
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Authors: | A Yu Aksenova K V Volkov N S Rovinsky A V Svitin L N Mironova |
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Institution: | (1) Department of Genetics and Breeding, St. Petersburg State University, St. Petersburg, 199034, Russia |
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Abstract: | Translation fidelity in Saccharomyces yeasts is determined by genetic and epigenetic (prion) factors. A study was made of S. cerevisiae strains containing the nonchromosomal determinant ISP
+], described earlier. Some of its properties suggest that ISP
+] is a prion. ISP
+] is expressed phenotypically as an antisuppressor of two sup35 mutations and can be cured with guanidine chloride (GuHCl). It was shown that sup35 mutants containing ISP
+] carried additional sup45 mutations. These mutations caused amino acid substitutions in different regions of translation termination factor eRF1, encoded
by SUP45. Strains bearing the sup35-25 mutation contained the sup45 mutation that caused amino acid substitution at position 400 of eRF1; strains bearing sup35-10 contained the mutation that altered eRF1 at position 75. Thus, the antisuppressor phenotype of the ISP
+] strains proved to depend on the interaction of sup35 and sup45 mutations, as well as on the GuHCl-curable epigenetic determinant.
Published in Russian in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 5, pp. 844–849.
The article was translated by the authors. |
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Keywords: | epigenetic inheritance yeast prions translation fidelity SUP45 SUP35 |
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