Plant regeneration,origin, and development of shoot buds from root segments of <Emphasis Type="Italic">Melia azedarach</Emphasis> L. (<Emphasis Type="Italic">Meliaceae</Emphasis>) seedlings |
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Authors: | Email author" target="_blank">Silvia?VilaEmail author Ana?Gonzalez Hebe?Rey Luis?Mroginski |
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Institution: | (1) Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, CC 209. (3400), Corrientes, Argentina |
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Abstract: | Summary
In vitro regeneration of plants from root culture of Melia azedarach seedlings was obtained. The origin and mode of development of the regenerated shoot buds were studied by means of histological
analysis and scanning electron microscopy (SEM). Maximum shoot bud regeneration was achieved when root segments were cultured
on Murashige and Skoog (MS) medium at quarter strength with 3% sucrose and 0.44 μM benzyladenine (BA) and kept under light (116 μmol m−2 s−1). Shoot bud elongation was achieved on MS with 0.44 μM BA, 0.46 μM kinetin (KIN), and 3.26 μM adenine sulphate (AD). Regenerated shoots were rooted on MS with 12.26 μM indole-3-butyric acid (IBA) for 4 d and subsequently in MS lacking plant growth regulators for 26 d. Plants were established
in a potting substrate. Histological analysis of roots from intact seedlings (without treatment) demonstrated that during
the early life of the roots, M. azedarach lacks preformed buds. In contrast, when the roots were excised and cultured in vitro, the histology and SEM observations revealed that buds originated from meristematic groups of cells, which had been formed
from the pericycle and several layers beneath. These meristematic groups of cells grew towards the periphery of the cortex
by crushing the outer layer of cortical cells. Further develoment led to the differentiation of leaf primordia and a shoot
apical meristem. |
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Keywords: | histology shoot buds root culture scanning electron microscopy |
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