| 肠道传播的非甲非乙型（戊型）肝炎病毒基因克隆的初步报告 |
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| 引用本文： | 毕胜利 曹学义. 肠道传播的非甲非乙型（戊型）肝炎病毒基因克隆的初步报告[J]. 病毒学报, 1991, 7(1): 66-68 |
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| 作者姓名： | 毕胜利 曹学义 |
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| 作者单位： | 中国预防医学科学院病毒学研究所(毕胜利，刘崇柏，汪媛，赵洪兰)，新疆维吾尔族自治区卫生防疫站(曹学义)，中国医学科学院实验动物研究所(王光明)，北京医科大学(庄辉) |
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| 摘 要： |
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| 关 键 词： | 非甲非乙肝炎 基因克隆 HEV |
A PRELIMINARY REPORT ON THE GENETIC CLONING OF HEPATITIS E VIRUS (HEV) |
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| Bi Shengli Liu Chongbai Wang Yuan Zhao Honglan Cao Xueyi Wang Guangming Zhuang Hui Institute of Virology,Chinese Academy of Preventive Medicine. Sanitary andAnti-epidemic Station,Xinjiang Autonomous. A PRELIMINARY REPORT ON THE GENETIC CLONING OF HEPATITIS E VIRUS (HEV)[J]. Chinese journal of virology, 1991, 7(1): 66-68 |
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| Authors: | Bi Shengli Liu Chongbai Wang Yuan Zhao Honglan Cao Xueyi Wang Guangming Zhuang Hui Institute of Virology Chinese Academy of Preventive Medicine. Sanitary andAnti-epidemic Station Xinjiang Autonomous |
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| Affiliation: | Bi Shengli Liu Chongbai Wang Yuan Zhao Honglan Cao Xueyi Wang Guangming Zhuang Hui Institute of Virology,Chinese Academy of Preventive Medicine. Sanitary andAnti-epidemic Station,Xinjiang Autonomous. Institute of Experimental Animal,Chinese Academy of Medical Sciences. Beijing Medical University |
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| Abstract: | Using the sequence independent single primer gene amplification method a 2,000bp 3' cDNA clone was isolated from the faeces collected from a acutely infected Chinese HEV patient and from the faeces, liver tissue of experimentally infected Rhesus monkey.The specificity of the clone isolated was confirmed by probes Hp1, Hp2, pF2 and pR3, which represented the more conservative sequences in p part of the HEV genome cloned from the faeces of a Burmese HEV patient.It was demonstrated that the SISpA products could be hybridized with all four probes,except that the pR3 probe gave a weaker reaction with the SISpA products. |
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| Keywords: | Hepatitis E Virus Sequence independent single primergene amplification method |
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