Immunoaffinity chromatography |
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Authors: | Anuradha Subramanian |
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Institution: | (1) 208 Biosystems and Agric. Engineering, Univ. Minnesota, 1390 Eckles Avenue, 55108 St. Paul, MN |
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Abstract: | Immunoaffinity chromatography is a process in which the binding affinity of an antigen to a parent antibody is utilized as
a basis of separation. Owing to the customized avidity and specificity, monoclonal antibodies (Mabs) have become indispensable
for both protein characterization and purification. The immunosorbent performance is dependent on the support matrix upon
which the antibody is immobilized and on the activation chemistry used couple the antibody to the matrix. This report details,
protocols to immobilize Mabs on commercially available supports, and a method to compute immunosorbent efficiency. |
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Keywords: | Monoclonal antibodies antigen immunosorbent coupling efficiency antigen-binding efficiency |
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