Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic. |
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| Authors: | J M van Noort P van den Berg I E Mattern |
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| Affiliation: | TNO-Medical Biological Laboratory, Rijswijk, The Netherlands. |
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| Abstract: | A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger. |
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