| 抗体在二氧化硅表面固定及活性测定 |
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| 引用本文： | 刘康栋,赵辉,金庆辉,赵建龙. 抗体在二氧化硅表面固定及活性测定[J]. 中国生物工程杂志, 2006, 26(6): 75-77 |
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| 作者姓名： | 刘康栋 赵辉 金庆辉 赵建龙 |
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| 作者单位： | 中国科学院上海微系统与信息技术研究所生物芯片室中国科学院上海微系统与信息技术研究所生物芯片室中国科学院上海微系统与信息技术研究所生物芯片室中国科学院上海微系统与信息技术研究所生物芯片室 |
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| 摘 要： | 应用分子自组装技术，通过表面羟基化、氨基硅烷化和对苯二甲醛组装，在二氧化硅表面衍生活泼的醛基基团。利用抗体的氨基和醛基发生还原胺化反应将抗体固定在二氧化硅表面，通过抗原抗体反应定性检测固定抗体的生物活性。结果显示应用这种方法能够有效将抗体固定在二氧化硅表面，并保持抗体生物活性，该固定方法在蛋白质检测、分析和蛋白质芯片中有广泛应用价值。
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| 关 键 词： | 蛋白质芯片 分子自组装 抗体固定 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-04-03 |
Antibody immobilization and activity investigation on silica surface |
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| LIU Kang-dong,ZHAO Hui,JIN Qing-hui,ZHAO Jian-long. Antibody immobilization and activity investigation on silica surface[J]. China Biotechnology, 2006, 26(6): 75-77 |
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| Authors: | LIU Kang-dong ZHAO Hui JIN Qing-hui ZHAO Jian-long |
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| Affiliation: | 1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences Shanghai 200050,China;2. The Graduate School of the Chinese Academy of Sciences Beijing 100039, China |
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| Abstract: | Aldehyde groups were produced on silica surface through silanisation and Terephthal-dicarboxaldehyde self-assembly. Antibody was immobilized on silica surface through condensation reaction between an amine and an aldehyde. Activity of antibody was investigated through antigen-antibody reaction. Experiment show antibody can be attached on silica surface through this way and its activity is retentive. This method can be used in protein detection and protein chip. |
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| Keywords: | Molecular self-assembly Antibody immobilization Protein chip |
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