Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection |
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Authors: | Christa Colyer |
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Institution: | (1) Department of Chemistry, Wake Forest University, 27109 Winston-Salem, NC |
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Abstract: | Interest in the use of capillary electrophoresis (CE) as a tool for protein separations continues to grow. Additionally, laser-induced
fluorescence (LIF) detection schemes promise ultrasensitive detection of small quantities of these important biomolecules
following their separation. In most cases, LIF detection of proteins necessitates their prior derivatization with a fluorescent
label molecule. To minimize the amount of additional sample handling and time associated with such labeling procedures, not
to mention the sometimes-stringent pH and temperature controls they require, noncovalent labeling is presented as a viable
alternative. This review article considers established methods for noncovalent labeling of proteins for their subsequent analysis
by CE-LIF. Label molecules suitable for excitation and emission in the ultraviolet, visible, and near-infrared regions of
the spectrum are enumerated for a variety of protein analytes. |
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Keywords: | Capillary electrophoresis laser-induced fluorescence CE-LIF protein noncovalent labeling |
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