Methodology for the analysis of fenbendazole and its metabolites in plasma, urine, feces, and tissue homogenates |
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| Authors: | S A Barker L C Hsieh C R Short |
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| Institution: | 2. University of Potsdam, Institute of Biochemistry and Biology, Potsdam, Germany;1. Departamento de Análises Clínicas Toxicológicas e Bromatológicas-Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil;2. Departamento de Bioquímica e Imunologia-Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil;3. Departamento de Imunologia-Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil;4. Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Brazil;5. Departamento de Parasitologia-Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil |
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| Abstract: | New methodology for the extraction and analysis of the anthelmintic fenbendazole and its metabolites from plasma, urine, liver homogenates, and feces from several animal species is presented. Quantitation of fenbendazole and its metabolites was conducted by high-pressure liquid chromatography using ultraviolet detection at 290 nm. The combined extraction and analysis procedures give excellent recoveries in all of the different biological matrices examined. High specificity, low limits of detection, and excellent linearity, accuracy, and inter- and intrasample variability were also obtained. The study of fenbendazole pharmacokinetics in vitro and in vivo should be greatly enhanced through the utilization of these methods. |
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