Dual effect of amino acids on the development of intracellular proteolytic activity in the irreversible sporulation phase ofBacillus megaterium |
| |
Authors: | Kučerová Helena Strnadová Marie Vinter Vladimír Votruba Jaroslav Chaloupka Jiří |
| |
Institution: | (1) Department of Molecular and Cell Microbiology, Institute of Microbiology, Czech Academy of Science, CZ-142 20 Prague, Czech Republic;(2) Department of Cell Structure and Metabolism, Institute of Microbiology, Czech Academy of Science, Prague, Czech Republic |
| |
Abstract: | Amino acids added to a population ofBacillus megaterium immediately after its transfer to a sporulation medium stimulated growth, delayed sporulation by 1 h, and delayed the development of intracellular cytoplasmic serine proteinase (ISP) activity. However, the ISP activity in late sporulation stages exceeded twice that of the control population. Amino acids supplemented at T3, i.e., at the time when engulfed forespores were developing, caused a decrease of specific ISP activity. The course of the phenylmethane sulfonyl fluoride (PMSF)-resistant activity in the cytoplasm was not affected by amino acids. Intracellular degradation of proteins prelabeled at the end of the growth phase was decreased by amino acids during the reversible sporulation phase but was only slightly affected later. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|