DEMONSTRATION AND PRELIMINARY CHARACTERIZATION OF REAGGREGATION-PROMOTING SUBSTANCES FROM EMBRYONIC SEA URCHIN CELLS |
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| Authors: | KAZUHIKO KONDO HIKOICHI SAKAI |
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| Institution: | Department of Biophysics and Biochemistry, Faculty of Science, Tokyo University, Hongo, Bunkyo-ku, Tokyo 112, Japan |
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| Abstract: | Morulae or early blastulae of sea urchins dissociate readily on treatment with isotonic urea containing EDTA. During dissociation a certain substance was extracted. This substance was found to accelerate reaggregation of dissociated cells. The manifestation of the reaggregation-accelerating activity of the substance requires rather complicated handling of divalent cations. On the basis of these findings, a working hypothesis on the mode of function of the substance is presented.
Some properties of the substance were investigated and were found to be very similar to those of hyalin, the calcium-insoluble protein of the hyaline layer surrounding the fertilized egg. |
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