Stain Technology: A Combined Elastic, Fibrin and Collagen Stain |
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Authors: | Winsome Garvey Arleen Fathi Francine Bigelow Blair Carpenter Carmencita Jimenez |
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Institution: |
a Children's Hospital of Eastern Ontario, Ottawa, Canada |
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Abstract: | A method is described which demonstrates nuclei, elastic fibers, red blood cells, collagen and fibrin. Nuclei and elastic fibers are stained by a modified VerhoefPs elastic tissue stain which was previously developed and used in the elastic-Masson combination. Both early fibrin and red blood cells are shown by Hssamine fast yellow. Mature fibrin, some types of collagen and other cytoplasmic changes are stained by a combination of acid fuchsia, Biebrich scarlet and ponceau 2R, while old fibrin is demonstrated by the collagen stain. This method takes about 1 hr to perform and has the added advantage that several entities are clearly shown in a single slide. |
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