Electrophoretic and molecular analysis of alpha-gliadins inAegilops species (Poaceae) belonging to the D genome cluster and in their putative progenitors |
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| Authors: | S. Masci R. D'Ovidio D. Lafiandra O. A. Tanzarella E. Porceddu |
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| Affiliation: | (1) Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, Via San Camillo de Lellis, I-01100 Viterbo, Italy |
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| Abstract: | The D genome cluster includes six allopolyploidAegilops species having as pivotal genome that ofAegilops squarrosa. Alpha-gliadins, endosperm proteins coded by multigenic families, have been analyzed in the D genome species cluster and in their putative progenitors. They can be present or weakly expressed when analyzed in acid polyacrylamide gel electrophoresis. Molecular analysis has shown the possibility to distinguish subsp.strangulata from subsp.eusquarrosa and to confirm the presence ofAe. caudata and ofAe. umbellulata in the polyploidsAe. cylindrica andAe. juvenalis, respectively. Finally, introgression fromAe. longissima orAe. searsii in tetraploid and hexaploidAe. crassa, Ae. juvenalis, andAe. vavilovii is supposed. |
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| Keywords: | Angiosperms Poaceae Aegilops Genome D cluster alpha-gliadins storage proteins phylogeny introgression electrophoresis restriction enzyme analysis |
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