Dissection of the functional structure of aptamer17, which specifically recognizes differentiated PC12 cells |
| |
Authors: | Liu Jiao-Jiao Wang Cheng-Long Xi Qing Xu Juan Deng Bin Ding Hong-Mei Chu Bingfeng Su Dong-Hua |
| |
Institution: | Department of Stomatology, General Hospital of PLA, Beijing, China. |
| |
Abstract: | A specific single-stranded DNA (ssDNA) aptamer (aptamer17) that specifically recognizes differentiated PC12 cells had been previously obtained after 6 rounds of whole cell-based subtractive systematic evolution of ligands by exponential enrichment selection from a random ssDNA library. To further investigate the relationship between the structure and function of this aptamer, 3 truncated ssDNA aptamers were designed according to the predicted secondary structure of aptamer17. Our results show that the stem-loop is the core structure of the aptamers required for specific binding to differentiated PC12 cells, specifically loops I and II. Aptamer17 and the truncated aptamers with this basic structure could bind specifically to differentiated PC12 cells and identify these cells from a mixture of differentiated and undifferentiated PC12 cells. Therefore, truncated forms of aptamer17 may be useful in the clinic to identify undifferentiated and differentiated PC12 cells from a mixture of cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|