Bacterial diversity of water and sediment in the Changjiang estuary and coastal area of the East China Sea |
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Authors: | Bi-Wei Feng Xiao-Ran Li Jin-Hui Wang Zi-Ye Hu Han Meng Ling-Yun Xiang & Zhe-Xue Quan |
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Institution: | Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China;and;East China Sea Environmental Monitoring Center, State Oceanic Administration, Shanghai, China |
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Abstract: | The Changjiang estuary and the coastal area of the East China Sea (ECS) represent important interfaces of terrestrial and marine environments. This study included analyses of water and sediments collected during different seasons in these regions to determine the composition of microbial assemblages by means of 16S rRNA gene clone libraries. We retrieved 1946 sequences and 779 distinct operational taxonomic units from 36 clone libraries. Shannon–Weaver diversity index values and rarefaction analysis indicated that bacterial diversity in the sediment samples was much higher than in the water samples. Proteobacteria (72.9%) was the most abundant phylum, followed by Firmicutes (6.4%), Bacteroidetes (4.6%) and Actinobacteria (4.1%). In the water, clone sequences related to Alphaproteobacteria were the most abundant, whereas in the sediment samples, sequences affiliated with Gammaproteobacteria were predominant. Principal coordinate analysis showed that water samples collected from the Changjiang estuary and the ECS clustered separately. However, this spatial pattern could not be observed in sediment samples, which were mainly distinguished from one another by the season. Bacterial diversity in the Changjiang estuary was higher than that in the ECS, which may be the result of the mixing of bacterial communities from the Changjiang River, the estuary and the coastal ocean. |
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Keywords: | bacterial diversity the Changjiang estuary the East China Sea (ECS) community structure clone library of 16S rRNA gene |
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