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Human cardiac myosin light chains: Sequence comparisons between myosin LC1 and LC2 from normal and idiopathic dilated cardiomyopathic hearts |
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| Authors: | John C Holt James B Caulfield Paul Norton Peter D Chantler Henry S Slayter Sarkis S Margossian |
| Institution: | (1) Rhône-Poulenc Rorer Central Research, 19406 King of Prussia, PA, USA;(2) Department of Pathology, University of Alabama, AL 35294 Birmingham, USA;(3) Unit of Molecular and Cellular Biology, The Royal Veterinary College, University of London, London, UK;(4) Dana-Farber Cancer Institute and Department of Cellular and Molecular Physiology, Harvard Medical School, 02115 Boston, MA, USA;(5) Departments of Biochemistry and Orthopedic Research Laboratories, Montefiore Medical Center and Albert Einstein College of Medicine, 10467 Bronx, N.Y., USA;(6) Division of Molecular and Cellular Medicine, Laboratory of Cardiac Biochemistry and Biophysics, Albany Medical College, 47 New Scotland Avenue, 12208 Albany, N.Y., USA |
| Abstract: | The primary structures of light chains isolated from the human myocardium with idiopathic dilated cardiomyopathy (IDC) were determined and compared with the sequence structures of myosin light chains obtained from control human heart myosin. Sequences were determined by chemical analysis and the identity of N-terminal residues established by mass spectrometry. The N-terminal residues in essential (ELC) and regulatory (RLC) light chains were blocked and were identified to be trimethyl alanine. The amino acid sequences of ELC and RLC from control human myosin revealed a high degree of homology with those purified from rat and chicken cardiac myosin. Comparison with a published partial chemical sequence of the human heart myosin light chains revealed significant variations. However, there was very good agreement with published sequences obtained by molecular biological techniques. Sequences of the light chains from cardiomyopathic myosin revealed no difference in the primary structures when compared with control human heart myosin light chains indicating IDC had no influence on, nor was caused by, altered myosin light chain gene expression. |
| Keywords: | cardiac myosin regulatory light chain essential light chain primary structure amino acid sequence idiopathic dilated cardiomyopathy |
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