Porcine blood cell separation by porous cellulose acetate membranes |
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Authors: | A Cenci? S Koren B Filipi? ? Stropnik |
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Institution: | A. Cenci?, S. Koren, B. Filipi? and ?. Stropnik |
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Abstract: | Leukocytes were separated from whole porcine blood using laboratory prepared polymeric asymmetric porous membranes from cellulose
acetate (CA) and by applying standard blood cell separation methods: centrifugation in a Ficoll solution gradient and in sucrose
solution concentration gradient. Leukocytes, obtained by different separation methods were characterised by their quantity,
type, viability and growth ability. Membranes prepared by a wet phase inversion process from different cellulose acetate/acetone/water
and magnesium chlorate VII systems, were characterised according to: permeability to deionised water, surface morphology and
by the determination of the flux of the permeate during the whole porcine blood separation. Cellulose acetate membranes prepared
from 300 μm thick cast solution (14.8 wt% of cellulose acetate, 19.9 wt% of water, 2.3 wt% of Magnesium perchlorate, and 63.0
wt% of acetone), have separation characteristics comparable with the standard separation methods; in the dead-end mode filtration,
21.3% of leukocytes from porcine whole blood are separated. The leukocyte number in peripheral blood before separation was
450,000 ml-1; the number passed through after was 95,000±6620. The main interest of the study was to introduce the CA membrane filters
for the continus technological separation of the leukocyte/lymphocytes from animal (= porcine, bovine, horse..) blood.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | cellulose acetate membranes leukocytes porcine blood separation |
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