The yeast Arf GTPase-activating protein Age1 is regulated by phospholipase D for post-Golgi vesicular transport |
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Authors: | Benjamin Jeremy J R Poon Pak P Lewis Stephen M Auger Andréanne Wong Tania A Singer Richard A Johnston Gerald C |
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Affiliation: | Department of Microbiology and Immunology, DalhousieUniversity, Halifax, Nova Scotia B3H 1X5, Canada. |
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Abstract: | Vesicular transport shuttles cargo among intracellular compartments. Several stages of vesicular transport are mediated by the small GTPase Arf, which is controlled in a cycle of GTP binding and hydrolysis by Arf guanine-nucleotide exchange factors and Arf GTPase-activating proteins (ArfGAPs), respectively. In budding yeast the Age2 + Gcs1 ArfGAP pair facilitates post-Golgi transport. We have found the AGE1 gene, encoding another ArfGAP, can in high gene-copy number alleviate the temperature sensitivity of cells carrying mutations affecting the Age2 + Gcs1 ArfGAP pair. Moreover, increased AGE1 gene dosage compensates for the complete absence of the otherwise essential Age2 + Gcs1 ArfGAP pair. Increased dosage of SFH2, encoding a phosphatidylinositol transfer protein, also allows cell growth in the absence of the Age2 + Gcs1 pair, but good growth in this situation requires Age1. The ability of Age1 to overcome the need for Age2 + Gcs1 depends on phospholipase D activity that regulates lipid composition. We show by direct assessment of Age1 ArfGAP activity that Age1 is regulated by lipid composition and can provide ArfGAP function for post-Golgi transport. |
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Keywords: | Endosomes G Proteins Golgi Membrane Trafficking Yeast Arf GTPase-activating Protein Arf1 Diacylglycerol Phosphatidylinositol Transfer Protein Post-Golgi Transport |
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