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干旱诱导性启动子驱动的海藻糖-6-磷酸合酶基因载体的构建及转基因烟草的耐旱性
引用本文:赵恢武,陈杨坚,胡鸢雷,高音,林忠平.干旱诱导性启动子驱动的海藻糖-6-磷酸合酶基因载体的构建及转基因烟草的耐旱性[J].Journal of Integrative Plant Biology,2000,42(6):616-619.
作者姓名:赵恢武  陈杨坚  胡鸢雷  高音  林忠平
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修稿时间:1999-08-11

Construction of a Trehalose-6-phosphate Synthase Gene Driven by Drought-responsive Promoter and Expression of Drought-resistance in Transgenic Tobacco
Authors:ZHAO Hui-Wu  CHEN Yang-Jian  HU Yuan-Lei  GAO Yin  LIN Zhong-Ping
Abstract:The trehalose-6-phosphate synthase gene (TPS) from Saccharomyces cerevisiae Hansen and the drought-responsive promoter from Arabidopsis thaliana (L.) Heynh. have been cloned by PCR procedure. A plant expression vector with TPS under control of Prd29A has been constructed and used for the genetic transformation of tobacco. The transgenic tobacco with Prd29A/TSP demonstrated TPS expression with increased drought tolerance under drought stress. Some obvious morphological changes including dwarf and fine shoot, lancet-shaped leaves and vigorous auxiliary buds have been observed in a few transformed plants.
Keywords:drought-responsive promoter    trehalose-6-phosphate synthase  Nicotiana tabacum  drought tolerance  
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