Liquid chromatography/mass spectrometry of fatty acids as their anilides |
| |
Authors: | T Kusaka M Ikeda H Nakano Y Numajiri |
| |
Affiliation: | Biochemistry Department, Kawasaki Medical School, Okayama. |
| |
Abstract: | The mass spectra of a series of saturated (C16:0-C30:0) and unsaturated (C16:1, C18:1, C18:2, C18:3) fatty acids have been recorded as their anilides using liquid chromatography/mass spectrometry with the atmospheric-pressure-ionization interface system. The spectra show an intense peak for (molecule + H)+ ion in each case. The liquid chromatographic/mass spectrometric separation was performed on a reverse phase column using a solvent system of methanol alone or methanol + 2-propanol. This method seemed promising for application to both qualitative and quantitative micro-analysis of fatty acids including very long chain fatty acids. |
| |
Keywords: | |
|
|