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Thin layer chromatographic analysis of glucose and maltose in estivated Biomphalaria glabrata snails and those infected with Schistosoma mansoni
Authors:Jarusiewicz Jamie A  Sherma Joseph  Fried Bernard
Institution:aDepartment of Chemistry, Lafayette College, Easton, PA 18042, USA;bDepartment of Biology, Lafayette College, Easton, PA 18042, USA
Abstract:Thin layer chromatography was used to analyze the glucose and maltose concentrations of the digestive gland–gonad complex (DGG) of uninfected-estivated Biomphalaria glabrata snails and estivated B. glabrata patently infected with Schistosoma mansoni. All snails were estivated in a most chamber at a relative humidity of 98 ± 1% and a temperature of 23 ± 1 °C for 14 days. Carbohydrates were extracted from the DGG with 70% aqueous ethanol, and extracts were analyzed on silica gel preadsorbent plates using ethyl acetate–glacial acetic acid–methanol–water (60:15:15:10) mobile phase, α-naphthol–sulfuric acid detection reagent, and quantification by densitometry. The concentrations of glucose and maltose were significantly reduced in both uninfected-estivated snails and infected-estivated snails.
Keywords:Biomphalaria glabrata  Densitometry  Estivation  Glucose  Maltose  Schistosoma mansoni  Thin layer chromatography
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