Thin layer chromatographic analysis of glucose and maltose in estivated Biomphalaria glabrata snails and those infected with Schistosoma mansoni |
| |
Authors: | Jarusiewicz Jamie A Sherma Joseph Fried Bernard |
| |
Institution: | aDepartment of Chemistry, Lafayette College, Easton, PA 18042, USA;bDepartment of Biology, Lafayette College, Easton, PA 18042, USA |
| |
Abstract: | Thin layer chromatography was used to analyze the glucose and maltose concentrations of the digestive gland–gonad complex (DGG) of uninfected-estivated Biomphalaria glabrata snails and estivated B. glabrata patently infected with Schistosoma mansoni. All snails were estivated in a most chamber at a relative humidity of 98 ± 1% and a temperature of 23 ± 1 °C for 14 days. Carbohydrates were extracted from the DGG with 70% aqueous ethanol, and extracts were analyzed on silica gel preadsorbent plates using ethyl acetate–glacial acetic acid–methanol–water (60:15:15:10) mobile phase, α-naphthol–sulfuric acid detection reagent, and quantification by densitometry. The concentrations of glucose and maltose were significantly reduced in both uninfected-estivated snails and infected-estivated snails. |
| |
Keywords: | Biomphalaria glabrata Densitometry Estivation Glucose Maltose Schistosoma mansoni Thin layer chromatography |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|