The degradation of arsenobetaine to inorganic arsenic by sedimentary microorganisms |
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Authors: | Hanaoka Ken'ichi Tagawa Shoji Kaise Toshikazu |
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Institution: | (1) Department of Food Science and Technology, Shimonoseki University of Fisheries, Yoshimi-cho, 759-65 Shimonoseki, Japan;(2) Kanagawa Prefectural Public Health Laboratories, Nakao-cho, Asahi-ku, 241 Yokohama, Japan |
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Abstract: | Two growth media containing arsenobetaine (CH3)3 As+ CH2COO–] were mixed with coastal marine sediments, the latter providing a source of microorganisms. The mixtures were kept at 25 °C in the dark and shaken for several weeks under an atmosphere of air. The disappearance of arsenobetaine and the appearance of two metabolites were followed by HPLC. The HPLC-retention time of the first metabolite agreed with that of trimethylarsine oxide (CH3)3AsO]. The second metabolite was identified as arsenate (As(V)) using hydride generation/cold trap/GC MS analysis and thin layer chromatography. This is the first scientific evidence showing that arsenobetaine is degraded by microorganisms to inorganic arsenic via trimethylarsine oxide. The degradation of arsenobetaine to inorganic arsenic completes the marine arsenic cycle that begins with the methylation of inorganic arsenic on the way to arsenobetaine. |
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Keywords: | arsenobetaine arsenic cycle degradation microorganisms sediment |
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