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Isolation,identification and localization of a second beetle antidiuretic peptide
Authors:Eigenheer Richard A  Wiehart Ursula M  Nicolson Susan W  Schoofs Liliane  Schegg Kathleen M  Hull J Joe  Schooley David A
Affiliation:Department of Biochemistry, 160 Howard Medical Science, University of Nevada, Mail Stop 330, Reno, NV 89557-0014, USA.
Abstract:We isolated from head extracts of Tenebrio molitor a peptide that inhibits fluid secretion by the Malpighian tubules of this insect. This second antidiuretic factor, ADFb, like the previously published ADFa, works through cyclic GMP as a second messenger. It has primary structure Tyr-Asp-Asp-Gly-Ser-Tyr-Lys-Pro-His-Ile-Tyr-Gly-Phe-OH with an EC(50) of approximately 240 pM in a fluid secretion assay. This peptide is now the second sequenced endogenous insect ADF which inhibits Malpighian tubule fluid secretion. Immunohistochemical techniques show that the peptide is localized in the brain; it appears to be produced mainly in two pairs of bilaterally symmetrical cells in the protocerebrum.
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