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| 冬虫夏草无性型—中国被毛孢固态发酵条件的初步研究 | |
| 引用本文： | 葛飞,桂琳,李春如,樊美珍.冬虫夏草无性型—中国被毛孢固态发酵条件的初步研究[J].生物学杂志,2009,26(3):22-25. |
| 作者姓名： | 葛飞 桂琳 李春如 樊美珍 |
| 作者单位： | 1. 安徽农业大学微生物防治安徽省重点实验室,合肥,230036;安徽工程科技学院生物与化学工程系,芜湖,241000 2. 皖南医学院微生物和免疫学教研室,芜湖,241001 3. 安徽农业大学微生物防治安徽省重点实验室,合肥,230036 |
| 基金项目： | 国家自然科学基金，安徽工程科技学院引进人才科研启动基金 |
| 摘 要： | 采用以麦角甾醇含量为指标间接测定固态发酵产物生物量的方法,对冬虫夏草无性型—中国被毛孢的固态发酵条件进行了研究。正交试验结果表明固态发酵最佳培养基组成为：大米5g,玉米粉2g,蚕蛹粉1g,麸皮2g;单因素试验结果表明：当培养温度20℃、料水比1：1.5、料层厚度2cm、无光照时对菌体生长较为有利。发酵条件优化后,菌丝体中麦角甾醇的含量可达0.5911mg/g,比优化前提高了38.6%。 |
| 关 键 词： | 冬虫夏草 中国被毛孢 固态发酵 培养基配方 |
Studies on solid-state fermentation condition of Hirsutella sinensis anamorph of Cordyceps sinensis |
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| GE Fei,GUI Lin,LI Chun-run,FAN Mei-zhen.Studies on solid-state fermentation condition of Hirsutella sinensis anamorph of Cordyceps sinensis[J].Journal of Biology,2009,26(3):22-25. | |
| Authors: | GE Fei GUI Lin LI Chun-run FAN Mei-zhen |
| Institution: | GE Fei,GUI Lin,LI Chun-run,FAN Mei-zhen(1.Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei 230036 ; 2. Department of Biochemical Engineering, Anhui University of Science and Technology, Wuhu 241000; 3. Department of Microbiology and immunology, Wannan Medical College, Wuhu 241001, China) |
| Abstract: | The optimum medium and optimal solid-state fermentation condition of Hirsutella sinensis, anamorph of Cordyceps sinen- sis, was investigated by the intracellular ergosterol content as an estimate of biomass. The results showed that the optimal condition was as follow ： silkworm pupa powder ： wheat bran ： corn meal ： rice at 1 ： 1 ： 2：7 temperature at 20℃, initial gradients-water ratio at 1 ： 1.5, medium thickness at 2 cm, and no illumination. The ergosterol content reached 0. 5911 mg/g in the mycelia fermented under optimized culture condition, improved by 38. 6% as compared with that under initial culture condition. |
| Keywords: | Cordyceps sinensis Hirsutella sinensis solid-state fermentation media components |
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