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Arachidonic-acid production by species of Mortierella
Authors:V K Eroshin  E G Dedyukhina  T I Chistyakova  V P Zhelifonova  C P Kurtzman  R J Bothast
Institution:(1) Institute of Biochemistry and Physiology of Microorganisms, 142292 Pushchino, Moscow Region, Russia;(2) Microbial Properties Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, 61604 Peoria, IL, USA;(3) Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, 61604 Peoria, IL, USA
Abstract:A growth-inhibiting, aspirin-containing medium was developed to select arachidonic-acid-(ARA)-producing Mortierella species and to determine the fatty-acid content of 87 Mortierella strains. ARA was detected in 66 strains from 33 species and its production may prove useful for systematic studies on Mortierella spp. The ARA content of the 66 producing strains tested ranged from 4% to 55% of total lipids. Most of the ARA-producing strains of Mortierella (59 strains) grown on potato/glucose/agar synthesized <20% ARA. Four strains produced between 20% and 25% ARA and three produced >40%. An inverse relationship was observed between ARA and oleic-acid contents.Names used in this article are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.
Keywords:Arachidonic acid  fatty acids  fungi  lipids  Mortierella
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