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Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factor |
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| Authors: | J R White P H Naccache T F Molski P Borgeat R I Sha'afi |
| Institution: | 1. Department of Physiology, University of Connecticut Health Center Farmington, CT. 06032 USA;1. Department of Pathology, University of Connecticut Health Center Farmington, CT. 06032 USA;7. Centre de Recherches en Endocrinologie Moleculaire Le Centre Hospitallier de l''Universite Laval, Saint-Foy, Quebec, Canada |
| Abstract: | An increase in the level of intracellular free calcium concentration in rabbit and human neutrophils stimulated by chemotactic factors has been demonstrated directly using the calcium-sensitive fluorescent probe quin-2. Addition of f-Met-Leu-Phe (10(-9) M), C5a (3 x 10(-9) M) or leukotriene B4 (6 x 10(-8) M) to the neutrophils induces a rapid increase in the intracellular concentration of free calcium that reaches a maximum value 15 seconds following stimulation. At concentrations of f-Met-Leu-Phe less than 10(-8) M the enhancement is dose dependent with an ED50 of 8 x 10(-11) M and is significantly reduced in the presence of EGTA in the suspending medium. |
| Keywords: | 5(S) 12(R) dihydroxy-6 8 11 14(cis trans trans cis) eicosatetraenoic acid HEPES Polymorphonuclear neutrophilic leukocytes neutrophils Formyl-Methionyl-Leucyl-Phenylalanine f-Met-Leu-Phe Ethyleneglycol-bis-(β-amino-ethyl-ether)N N′ -tetra-acetic acid EGTA Nanometer nm Nanomolar >nM |
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