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Effectiveness of acylation of protein amino groups with fatty acid chlorides in a system of reversed micelles of aerosol OT in octane
Authors:A V Kabanov  A L Klibanov  V P Torchilin  K Martinek  A V Levashov
Abstract:The characteristics of water-soluble enzyme (alpha-chymotrypsin) modification with 3H] palmitoyl chloride in the reversed Aerosol OT micelles in octane were determined. The degree of enzyme modification depends on the molar ratio palmitoyl chloride]/protein]. The modification reaction is characterized by the wide pH-optimum range and proceeds with high speed.
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