The rate of initiation of alpha- and beta-globin mRNA translation is modulated by 50 kDa, 28-kDa and 24-kDa polypeptide-containing fractions |
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Authors: | A Parets Soler L Reibel G Schapira |
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Institution: | Institut de Pathologie Moléculaire, INSERM U.15 24, rue du Faubourg Saint-Jacques, 75014 PARIS (France) |
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Abstract: | The relative rates of initiation of alpha- and beta-globin mRNA translation in a Krebs II ascites cell-free system are differently modulated by a 50-kDa protein and two fractions containing either a 28-kDa or a 24-kDa polypeptide. Each of these fractions stimulated a discrete step that limits initiation of protein synthesis, but other rate-limiting steps take place upstream and/or downstream, resulting in characteristic kinetics of the stimulation of alpha- and beta-globin synthesis. The ascites extracts appear to be deficient in these activities. |
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Keywords: | 7-methylguanosine 5′ triphosphate PCF polypeptide-containingfraction |
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